Untangling adaptive functioning of PMM2-CDG across age and its impact on parental stress: a cross-sectional study

Phosphomannomutase deficiency (PMM2-CDG) leads to cerebellar atrophy with ataxia, dysmetria, and intellectual deficits. Despite advances in therapy, the cognitive and adaptive profile remains unknown. Our study explores the adaptive profile of 37 PMM2-CDG patients, examining its association with parental stress and medical characteristics. Assessment tools included ICARS for the cerebellar syndrome and NPCRS for global disease severity. Behavioral and adaptive evaluation consisted of the Vineland Adaptive Behavior Scale and the Health of the Nation Outcome Scales. Psychopathological screening involved the Child Behavior Checklist and the Symptom Check-List-90-R. Parental stress was evaluated using Parental Stress Index. Results were correlated with clinical features. No significant age or sex differences were found. ‘Daily living skills’ were notably affected. Patients severely affected exhibited lower adaptive skill values, as did those with lipodystrophy and inverted nipples. Greater severity in motor cerebellar syndrome, behavioral disturbances and the presence of comorbidities such as hyperactivity, autistic features and moderate-to-severe intellectual disability correlated with greater parental stress. Our study found no decline in adaptive abilities. We provide tools to assess adaptive deficits in PMM2-CDG patients, emphasizing the importance of addressing communication, daily living skills, and autonomy, and their impact on parental stress in clinical monitoring and future therapies.

The VABS-3 is a caregiver-completed assessment that measures adaptive behavior.It yields standard scores in different domains: communication, daily living skills, and socialization.These domain scores are combined to generate an overall Adaptive Behavior Composite (ABC) score.Higher scores indicate better adaptive functioning, while lower scores suggest challenges in adaptive behavior.Additionally, equivalent developmental age can be calculated.Since the VABS3 is designed to measure adaptive behavior of individuals from birth to age 90, for analytical purposes, three age groups were defined ad hoc: children (6 years of age to under 12 years of age), adolescents (12 years of age to under 18 years of age), and adults (over 18 years of age).They were evaluated separately in three severity groups defined in this case as severely affected if their ABC score was below the median value of the sample and age group.Conversely, individuals were categorized as mildly affected if their ABC score was above that median value.
The whole group was evaluated using the HoNOSCA for children and the HoNOS-LD for adults, as measures of general health and social functioning 22,24,25 .

Psychopathological screening
The CBCL is a widely used caregiver report for evaluating behavioral, emotional, and social problems in children and adolescents.The scale sets provide scores associated with disorders from the Diagnostic and Statistical Manual of Mental Disorders 27 , including affective problems, anxiety problems, somatic problems, attention deficit with hyperactivity (ADHD), oppositional defiant problems and conduct problems, demonstrating good reliability when compared to psychological diagnoses 28 .
To screen adults for a range of psychopathological features, the SCL-90R, a multidimensional questionnaire for people with learning disabilities and mental health needs, was applied 26 .

Parental stress
PSI-SF is a short test designed to evaluate the magnitude of stress in the parent-child system 21 .It includes self-report questionnaire about three major domains: Parental Distress, Parent-Child Dysfunctional Interaction, and Difficult Child, which combines to form a Total Stress scale.After obtaining the direct scores, a percentile score can be obtained.Scores 85th percentile or higher are considered clinically significant.

Data analysis
Descriptive statistics were calculated for numerical variables, and frequency tables were generated for categorical variables.Normality in numerical variables was assessed with QQ plots.Differences in numerical variables between two groups were studies with Student's t test or Mann-Whitney's U test, and ANOVA or Kruskall-Wallis tests were used in comparisons between more than 2 groups.Tukey's test or pairwise Mann-Whitney's U test were performed as post-hoc analysis for ANOVA and Kruskall-Wallis tests, respectively.Effect sizes were calculated for these tests using Cohen's d (for Student's t test), rank-biserial correlation (r) (for Mann-Whitney's U test), η 2 (for ANOVA) and η 2 (H) (for Kruskal-Wallis test).The relationship between categorical variables was studied with Chi-square test or Fisher's exact test.Pearson and Spearman's correlations were used to study the relationship between numerical variables.Correlation coefficients above 0.7 were considered strong correlations, while values between 0.5 and 0.7 are considered moderate correlations.All statistical tests were two-sided.P values < 0.05 were considered significant.Statistical analysis was carried out using SPSS V.23.0 (Armonk, NY: IBM Corp.).

Ethical issues
All procedures followed were in accordance with the ethical standards of the responsible committee on human experimentation (institutional and national) and with the Helsinki Declaration of 1975, as revised in 2000.Ethical permission for the study (Internal Approval Code: PIC-108-14) was obtained from the Research & Ethics Committee of the SJD Research Foundation (https:// www.irsjd.org/ en/ resea rch/ ceim/).Parents gave their written informed consent, and children/adolescents and adults gave their assent.For children below 16 years of age, informed consent was obtained from all subjects and/or their parents/legal guardian.Adult patients gave their written informed consent when possible.Samples were obtained in accordance with the Helsinki Declaration of 1964, as revised in October 2013 in Fortaleza, Brazil.

Results
Thirty-seven individuals affected by PMM2-CDG were evaluated.Twenty-one were children (mean age 12.4 years; standard deviation (SD) 3.8; twelve males, nine females), and 16 were adults (mean age 29.3 years; SD 8.0; nine males, seven females).Individuals' characteristics and previous diagnoses in the neurodevelopmental area are presented in Table 1.For mental health monitoring, in children, three out of 21 had regular psychologist visits, and two visited both psychologists and psychiatrists.In adults, two out of 16 had regular psychiatrist follow-ups, and another one visited a psychologist regularly.
The mean NPCRS value for children was 18.3 (SD 7.5), corresponding to a moderate categorization as a group.In fact, nine of them were classified as moderate.For adults, the mean NPCRS value was 17.5 (SD 4.5), with eleven of them categorized as moderate.Out of 21 children, eight were unable to complete the ICARS scale due to comprehension difficulties.Thirteen children were evaluated, with a mean ICARS score of 43.4 over 100 (SD 25.2).In the adult group, three individuals were unable to perform the ICARS maneuvers, and two could not be evaluated.Eleven ICARS assessments were conducted, resulting in a mean score of 38.

Adaptive behavior and functioning
With regard to VABS-3, there were no statistically significant differences between sex and age groups, even when considering the split of the sample of children into young children and adolescents.VABS-3 results of young children (n = 7), adolescents (n = 12) and adults (n = 16) are presented in Fig. 1.As domains, 'Daily living skills' was the most severely affected specific domain.For both age groups all domains and the ABC score were affected (all mean values were in the low range) (Table 2).Considering subdomains, 'Domestic' in the domain 'Daily living skills' , and 'Coping skills' in the 'Socialization' domain, were the most affected across the ages (Fig. 1A).There was a general improvement with age in all the domains.Conversely, when the evaluation was performed clustering patients into age and severity groups,  Both severely affected adolescents and adults present equivalent ages that are lower than or close to five years of age.In contrast, the mildly affected groups show some improvement in the subdomains as they age, although they still do not reach the expected age level.
the most severely affected adolescents and adult patients maintained an adaptive functionality level under that for an equivalent age of 6 years (Fig. 1B,C and D).Lower scores in three different domains were associated with the presence of epilepsy ('Communication skills' , 'Daily living skills' , and ' ABC score'), and individuals with inverted nipples showed lower scores in all the domains.Also, lipodystrophy was related to lower scores in the four domains (Fig. 2).There were no other statistically significant associations with laboratory findings or medical features, including cerebellar measure.
There was a relation between NPCRS severity categories and abnormal scores in all VABS-3 domains (Communication domain p = 0.002, η 2 = 0.34; Daily living skills domain p = 0.001, η 2 = 0.37; Socialization domain p = 0.03, η 2 = 0.19; and ABC score p = 0.004, η 2 = 0.31).No correlation between VABS-3 domain results and raw scores in total NPCRS was found.However, scores in sections I and III of the NPCRS showed negative significant correlations with 'Communication' , 'Daily living skills' and ' ABC score' domains in children.Total score in section I also shed negative significant correlation with 'Communication' , 'Daily living skills' and ' ABC score' domains in adults (Table 2).For ICARS, no statistical correlations were found with the VABS-3 domain results, but those patients unable to complete an ICARS evaluation due to intellectual disability presented significantly lower mean scores in all VABS-3 domains.Concerning the HoNOSCA results, there were no differences among sex groupings.HoNOSCA identified moderate-to-severe problems in more than 50% of the children in the following areas: 'Scholastic or language skills' (95.2%), 'Physical illness or disability problems' (76.2%), 'Self-care' (76.2%), 'Non-organic somatic symptoms' (symptoms that do not have an identifiable organic or medical cause, where psychological or emotional factors may be underlying) (66.7%), and 'Overactivity attention and concentration' (52.4%) (Supplementary Table 1).
There were statistically significant associations between severity measured with NPCRS and HoNOSCA in the areas with identified problems (Supplementary Table 1).The presence of dysmorphic traits such as characteristic gestalt, inverted nipples, and lipodystrophy were each statistically associated with pathological scores in vs. 48.9,p = 0.001), 'Socialization' (88.9 vs. 62.0, p = 0.015), and ' ABC score' (86.0 vs. 60.6,p = 0.007).Regarding HoNOS tests, characteristic gestalt, inverted nipples, and lipodystrophy were, each statistically associated with pathological scores in 'Physical illness' , and 'Self-care' item, both in children and adults.ANOVA test showed a significant relation between NPCRS severity categories and abnormal scores in VABS-3 domains: 'Communication' , 'Daily living' , 'Socialization' , and ' ABC score' .Chi square test showed statistically significant associations between NPCRS and the altered areas in the HoNOS tests.The absence of lipodystrophy was related to an increase in significant scores for 'Withdrawn/depressed' (p = 0.04) and also ' Affective problems' (p = 0.035) in the CBCL evaluation.There was a positive Spearman correlation between the higher ICARS results and the highest Parental Distress domain scores (r: 0.648, p = 0.031).Higher scores in the total stress scale on the PSI correlated with higher scores in four items on the HoNOSCA: 'Disruptive, antisocial or aggressive behavior' (r: 0.615, p = 0.003), 'Non accidental self-injury' (r: 0.624, p = 0.002), and 'Emotional and related symptoms' (r: 0.738, p = 0.000).There was a statistically positive association between significant scores in the Parent-Child Dysfunctional Interaction item and significant scores at the CBCL (' Aggressive and rule-breaking behavior' and 'Oppositional and defiant disorder') and a correlation between scores in 'Difficult child' and 'Total stress' and some CBCL scores, showing a Pearson's r greater than 0.7 in all cases.
the HoNOSCA scale in different items (Fig. 2, Supplementary Table 1).There was no relation between significant scores and the listed laboratory findings or medical problems.
Regarding the results of HoNO'S-LD for adults, it also identified moderate-to-severe problems in more than 50% of the patients in the following areas, in order of prevalence: ' Activities of daily living at home' and 'outside the home' (81.3%), 'Physical problems' (62.5%), and 'Occupation and activities' (56.3%).There were no differences between the sexes.The higher severity seen through the NPCRS was associated with higher results in the ' Activities of daily living at home' and 'outside the home' .As with children, the presence of inverted nipples and lipodystrophy was statistically associated with pathological scores in different HoNO'S-LD items in the adult group (Supplementary Table 2).

Psychopathological screening
The assessment of behavioral emotional and social problems using the CBCL showed that 'Social problems' , 'Internalizing problems' and ' Attention problems' were the most significant, including around 50% of individuals in Limit or Significant values (Table 3).The 'Total Problems' score also yielded more than 50% of the subjects with scores in the limit or significance range, denoting a high level of overall behavioral and emotional difficulties.
Regarding sex, there were no differences, with the only exception being ' Anxiety problems' , which were more prevalent in males (p = 0.045, Cohen's d = 0.95).The absence of lipodystrophy was associated with an increase in significant scores for 'Withdrawn/depressed' and also ' Affective problems' .There was no other statistically significant relation with other dysmorphic traits, laboratory findings, clinical evaluations or medical problems.
On evaluating the psychological symptoms and psychopathological features of adults using the SCL-90R, no differences between the sexes were found.No relevant comorbidities were evident, with only the Positive Distress item significant in four out of 16 patients (Supplementary Table 3).

Parental stress
Regarding the magnitude of stress assessed through the PSI-SF, a high prevalence of clinically significant results (near 50% or greater) was found, both in the 'Parental Distress' items focused on parental feelings of distress, such as anxiety, depression, and feeling overwhelmed, and also those 'Parent-Child Dysfunctional Interaction' items exploring issues like discipline problems, attachment difficulties, and disrupted communication in the parent-child relationship.There were no statistical differences between age or sex groups.Although not significant, 'Defensive response' and 'Parental distress' showed the highest results in children (Table 4).High scores were found in the 'Defensive response' domain, which evaluates whether parents are being completely forthcoming in their responses.Parents of five children and three adults had high or clinically significant results in all the items of PSI-SF, meaning a score above the 85th percentile.All of those patients presented a formal diagnosis of mental health disorder such as ADHD, autism spectrum disorder (ASD) and/or, moderate-to-severe intellectual disability (Fig. 2).
There were no statistically significant associations between the patients' severity measured with the NPCRS, laboratory findings, clinical characteristics or the VABS-3 assessment and results in PSI-SF scores.In contrast, there was a positive correlation between the higher ICARS results and the higher Parental Distress domain scores.Table 3. Child behavior checklist (CBCL) assessment results.Problems showing limit or significant scores in more than 30% of the sample are in bold.SD Standard deviation; IQR Interquartile range.*Higher scores in these items were statistically associated with higher scores in PSI in the following domains: Parental distress, Parent-child dysfunctional interaction, difficult child, total stress.

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There was a statistically positive association between significant scores in several PSI-SF items and the significant scores on the CBCL and the HoNOSCA (Fig. 2, Table 3 and 4).

Discussion
PMM2-CDG is the best-known type of CDG.However, this is the first study to evaluate the adaptive behavior, functioning and psychopathological profile of PMM2-CDG patients, something of significant concern for families.Intellectual difficulties, problems with daily activities and self-direction, along with behavioral disturbances, limit patients' autonomy and negatively impact the quality of life of both patients and their families 12 .However, the assessment of these clinical behavioral and cognitive characteristics is not typically included in physician evaluations or clinical trial protocols, and no tools have been specifically tested in this patient group.Although, developmental delay is common in PMM2-CDG patients, intellectual disability is not always present 4,10 .Regarding learning disabilities, HoNOSCA results indicated that scholastic difficulties were present in almost all the patients.In the child group, more than 50% received a formal ADHD diagnosis, although we believe that comprehensive evaluation of executive functions was not conducted for the entire sample, particularly for those in the adult group.Additionally, the presence of an intellectual disability can hinder formal diagnosis of ADHD, even though it is often observed in genetically determined neurodevelopmental conditions 29 .ASD is also very prevalent in this cohort, having been diagnosed in almost one third of patients.Nevertheless, the presence of ASD traits (lack of cognitive flexibility, difficulties in socialization, and lack of skills in communication apart from speech) not fulfilling all the ASD criteria and not leading to a formal diagnosis, but still deserving of attention, is probably greater, as is the case in other neurogenetic conditions 29 .Importantly, the cerebellum has been identified as playing a significant role in both executive and emotional functions, potentially contributing to the features observed in ADHD and ASD [7][8][9] .
In our PMM2-CDG sample, no significant correlations were found between cerebellar atrophy and adaptive functioning or psychopathological findings in contrast with a previous report 30 .As a major limitation, it should be noted that the neuroimaging studies were evaluated retrospectively; they were obtained at very different ages, and, it is likely that as significant atrophy becomes more pronounced with age and residual volume is affected, the ability to differentiate between phenotype severities with imaging may diminish 16 .
VABS-3 has been widely used to describe the adaptive phenotype of neurodevelopmental conditions and inborn errors of metabolism, and also as a tool to evaluate the efficacy of interventions 31,32 .Our study shows that all the domain scores and their equivalent developmental ages were low, for both age groups.The domain 'Daily Living Skills' was the most severely affected, indicating difficulties in performing age-appropriate everyday tasks, including personal, domestic, and community activities.The next most affected domain was 'Communication' .Communication difficulties are a frequent source of distress for both parents and patients, often leading to frustration associated with challenges in expression and comprehension.
When evaluating by subdomains, two were consistently lower than the others across the sample: 'Domestic' (household task performance) and 'Coping skills' (how the individual demonstrates behavior and emotional control in different situations involving others), directly impacting autonomy and functionality.Although our study design is not longitudinal, it shows general improvement across the different age groups in all the domains.But evaluating mildly and severely affected patients as a different subset, it is seen that, across the different age groups of severely affected patients, there is little adaptive skills improvement; they maintained an adaptive functionality under an equivalent age of six years.Conversely, in mildly affected patients, adaptive skills exhibit The presence of epilepsy, the categorization of the NPCRS as severe, and higher values in section I of NPCRS, but also section III in the case of children, were associated with lower scores in VABS-3.Also, higher NPCRS categorization was associated with pathological scores in different areas of HoNOS scales, denoting a consistency among the different evaluations.
Inverted nipples and lipodystrophy were highlighted as severity signs in a previous study 10 .The group of subjects presenting them showed statistically significant lower scores in almost all the domains and subdomains of VABS-3 (Fig. 2), denoting difficulties in adaptive behavior, but also abnormal scores in different HoNOS scales items (Supplementary Tables 1 and 2), again highlighting their significance as markers of severity.Together with epilepsy, they may indicate a more severely affected subset of patients in terms of adaptive functioning.The association between clinical severity, adaptive functioning limitations, and dysmorphic traits in PMM2-CDG is likely grounded in the underlying mutations.These mutations might impact both physical features and the overall clinical presentation, establishing the connection between the early dysmorphic traits and the global severity of the condition.Today, given the absence of a known genotype-phenotype correlation, the presence of lipodystrophy and inverted nipples, both detected early in the newborn or infant, probably points towards greater adapting functioning limitations and, latterly, a more severe neurological phenotype.
In summary, VABS-3 and HoNOS scales point up the difficulties in daily living activities, particularly domestic tasks, as well as coping skills, but also in scholastic, occupational and working tasks, alerting to a severe impact on patients' autonomy and social relationships caused by the disease.
CBCL results showed significant social difficulties and attention problems, affecting around 50% of individuals.These difficulties appeared to be related to communication and executive functions, as indicated by the VABS-3 assessment.Unfortunately, this study lacks a formal assessment of executive functions, despite their significance as an abnormal domain in both CBCL and VABS-3.
Surprisingly, anxiety problems were found to be more prevalent in boys, which is in contrast to the general population 33 , what is described in intellectual disability groups 34 , and to a recently reported PMM2-CDG series 30 , all showing more risk of developing anxiety in the female group.Emotional problems and depression were not found to be relevant problems by any of the screening tools.Another recent study, by Ligezka et al. 35 , collecting patient-reported outcomes, did not find differences on anxiety and depression domains in PMM2-CDG patients compared to the general population, but the analysis of PROMIS domain T-scores by age showed higher anxiety and depression scores in patients older than 18 years compared to younger patients, and the analysis by gender showed higher anxiety and depression symptoms in women.Remarkably, a subset of patients with absence of lipodystrophy showed a statistically significant correlation to an increase in the existence of significant 'Withdrawn/depressed' and also ' Affective problems' .In an attempt to explain this apparently incongruent fact, if lipodystrophy has been previously described as a severity indicator 10 , patients who do not present it may be more conscious of their difficulties and therefore it has a greater impact on their emotional situation and self-esteem.
PSI-SF evaluates stress in the parent-child system, which is expected to some extent.A notable finding was the high scores in the 'Defensive response' domain, which evaluates whether parents are being completely forthcoming in their responses.This high score denotes the tendency to minimize or normalize the real impact of stress in their lives.The 'Parental distress' domain, reflecting difficulties in adjusting to parenthood, showed the highest scores, particularly in children, highlighting the importance of finding ways to reduce distress through community and social resources.Interestingly, higher ICARS scores, as a sign of motor cerebellar syndrome, correlated with higher scores in the 'Parental distress' domain.Behavioral disturbances identified by the CBCL are associated with more pathological scores on most items, and the presence of comorbidities such as ADHD, ASD, and moderate-to-severe intellectual disability is also relevant, as all individuals with clinically significant results in all PSI-SF items have one of these neurodevelopmental disorders.In conclusion, both motor impairments and behavioral complexity contribute to increased burden and distress among caregivers.Importantly, parenting interventions may ameliorate the family burden but also favor the parent-child interaction.

Conclusions
Our study highlights different areas of adaptive deficits that need to be prioritized in future intervention programs and therapies.'Daily living skills' was the most severely affected domain, both in the VABS-3 and HoNOS tests, directly impacting autonomy.'Communication' domain was also clearly affected.Evaluation of self-sufficiency and functionality, which may alter school activities and occupational tasks but also leisure, deserve attention as has been overlooked in clinical papers on PMM2-CDG.
Our study introduces validated tools available in different languages and widely recognized in the field for assessing and monitoring adaptive functioning.We suggest the use of VABS-3 in the regular patients' evaluation since it offers a comprehensive assessment of adaptive functioning and daily living skills, crucial domains affected in PMM2-CDG.The study shows external correlation of the tests with clinical indicators of severity, lending support to their use in the clinical practice.These tools also include a standardized assessment of the family's stress burden.Considering their high impact on autonomy, adaptive functioning, psychopathological risks, and family stress should be taken into account in evaluation, monitoring, and intervention programs for PMM2-CDG patients, including therapies under development.

Figure 1 .
Figure 1.VABS-3 equivalent developmental ages and domains.The equivalent developmental age for three age groups: Adults (green), adolescents (12-18 years, yellow), and children (5-11 years, blue).Patients under 5 have been excluded to facilitate homogeneity.(A) Shows the mean values of equivalent developmental age for every age group in the different subdomains.The graph shows an increase in equivalent developmental age across all domains.The subdomain 'Domestic' (domain 'Daily living skills') is severely affected in all age groups not reaching nine years of equivalent developmental age.This subdomain indicates the individual's abilities and independence in performing everyday tasks according to the individual's age and related to household activities and routines (personal hygiene, meal preparation, cleaning, managing personal belongings, etc.).The other severely affected subdomain is 'Coping skills' (domain 'Socialization'), also consistently affected across the ages and related to an individual's ability to demonstrate behavior and emotional control in different situations involving others.(B) and (C) show the equivalent developmental age in different subdomains for adolescents and adults including the mean scores (continuous line), the mean scores of the severely affected (those with ABC score below the median value, scores represented by the dotted lines) and the mean scores of the mildly affected (those with ABC score above the median value, scores represented by the dashed lines).(D) Further illustrates the equivalent developmental age for severely affected adolescents and adults (represented by the dotted lines), mildly affected adults and adolescents (represented by the dashed lines) and the group of children.Both severely affected adolescents and adults present equivalent ages that are lower than or close to five years of age.In contrast, the mildly affected groups show some improvement in the subdomains as they age, although they still do not reach the expected age level. https://doi.org/10.1038/s41598-023-49518-ywww.nature.com/scientificreports/

Figure 2 .
Figure 2. Assessments results and their interrelations.The altered areas or domains are highlighted using an exclamation sign.Statistically significant relations between the assessments are marked using red arrows.*Dysmorphic features evaluated were typical facial gestalt, lipodystrophy and inverted nipples.Student T test showed lower scores in different VABS-3 areas: communication skills (mean 70.2 vs. 43.2,p = 0.006), daily living skills (63.0 vs. 39.8, p = 0.007), and ABC score (75.1 vs. 48.7,p = 0.001).Individuals with inverted nipples showed lower scores in all the domains: 'Communication' (83.8 vs. 54.0,p = 0.004), 'Daily living' (75.6 vs. 48.4,p = 0.002), 'Socialization' (88.1 vs. 60.2, p = 0.006), and ' ABC score' (82.7 vs. 59.9, p = 0.009).Lipodystrophy associated lower scores in the four domains: 'Communication' (mean 88.4 vs. 54.8,p = 0.002), 'Daily living' (80.6vs.48.9, p = 0.001), 'Socialization' (88.9 vs. 62.0, p = 0.015), and ' ABC score' (86.0 vs. 60.6,p = 0.007).Regarding HoNOS tests, characteristic gestalt, inverted nipples, and lipodystrophy were, each statistically associated with pathological scores in 'Physical illness' , and 'Self-care' item, both in children and adults.ANOVA test showed a significant relation between NPCRS severity categories and abnormal scores in VABS-3 domains: 'Communication' , 'Daily living' , 'Socialization' , and ' ABC score' .Chi square test showed statistically significant associations between NPCRS and the altered areas in the HoNOS tests.The absence of lipodystrophy was related to an increase in significant scores for 'Withdrawn/depressed' (p = 0.04) and also ' Affective problems' (p = 0.035) in the CBCL evaluation.There was a positive Spearman correlation between the higher ICARS results and the highest Parental Distress domain scores (r: 0.648, p = 0.031).Higher scores in the total stress scale on the PSI correlated with higher scores in four items on the HoNOSCA: 'Disruptive, antisocial or aggressive behavior' (r: 0.615, p = 0.003), 'Non accidental self-injury' (r: 0.624, p = 0.002), and 'Emotional and related symptoms' (r: 0.738, p = 0.000).There was a statistically positive association between significant scores in the Parent-Child Dysfunctional Interaction item and significant scores at the CBCL (' Aggressive and rule-breaking behavior' and 'Oppositional and defiant disorder') and a correlation between scores in 'Difficult child' and 'Total stress' and some CBCL scores, showing a Pearson's r greater than 0.7 in all cases.

Table 1 .
Cohort description: epidemiological, laboratory and clinical description.

Table 2 .
Domains and subdomains of the VABS-3: means, interquartile ranges and ranges with age equivalents.Vineland descriptors: high (scores of 130-140), moderately high (scores of 115-129), adequate (scores of 86-114), moderately low (scores of 71-85) and low (scores of 20-70); yrs: years.SD Standard deviation; IQR Interquartile range; ABC Adaptive behavior composite; SI Section I of NPCRS; SIII Section III of NPCRS.*Using Spearman test, only significant moderate or strong correlations with the NPCRS sections are shown: SI for section I, SII for section II, and SIII for section III.

Table 4 .
Parenting stress index, fourth edition short form.SD Standard deviation; IQR Interquartile range.*Concerningparents' answers, 85th percentile and above are considered clinically significant scores.improvementacross age groups.Additional research employing a longitudinal design is imperative to substantiate the observed trend of stabilization or improvement with age, particularly within a subset of patients.